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Date of Award

2017

Document Type

Restricted Thesis: Campus only access

Degree Name

Bachelor of Science

Department

Biology

First Advisor

Dr. Krista Stenger

Abstract

The NF-kB pathway is vital for immune system regu- lation and pro-inflammatory signaling. Many disor- ders and diseases, including cancer, can be linked to NF-kB dysregulation. When macrophages recognize the presence of a pathogen, the signaling pathway is activated - resulting in the nuclear translocation of NF- kB to turn on pro-inflammatory genes. Here, we demonstrate the effects of a novel microtubule depol- ymerizer, NT-07-16, a polysubstituted pyrrole on this process. Treatment with NT-07-16 decreased the pro- duction of pro-inflammatory mediators in a dose-de- pendent manner in RAW264.7 mouse macrophages. It appears that the reduction in pro-inflammatory me- diators by the macrophages after exposure to NT-07- 16 may be due to a decrease in the translocation of NF- κB into the nucleus. Therefore, this study suggests that, upon activation of mouse macrophages, NF-kB translocates into the nucleus by way of the microtu- bule network and that disruption of this network by NT-07-16 reduces the inflammatory activity of the macrophages.

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