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Date of Award
4-28-2006
Document Type
Restricted Thesis: Campus only access
Degree Name
Bachelor of Science
Department
Biology
First Advisor
Dr. Valerie Kish
Abstract
Forty-four percent of all cancers of the central nervous system are malignant gliomas, and of these, at least 23% are glioblastoma multifonne (GBM). Different GBM cell lines have varying p53 gene status and produce varying amounts of proteins. One of the most significant proteins produced is matrix metalloproteinase 2 (MMP-2), which is associated with degradation, of the extracellular matrix (ECM). This allows tumor cells to move throughout the brain. The purpose of these experiments was to determine how MMP-2 activation was altered in the presence of Epidermal Growth Factor (EGF) and epigallocatechin gallate (EGCG) within U87 (wild type for p53) and T98 (mutant for p53) cancer cell lines. Data obtained suggested that U87 cells produced dose-dependent decreases in active MMP-2 in the presence of EGF, as well as showing a clear decrease in activation with EGCG treatment. The T98 cell line showed a slight decrease in activation when treated with EGCG but showed no change with EGF treatment.
Recommended Citation
Boucher, Catherine, "The effects of epidermal growth factor and the green tea flavanoid, epigallocatechin-3-gallate, on MMP-2 activation in human glioma cell lines U87 and T98" (2006). Honors Theses. 341.
https://scholarship.richmond.edu/honors-theses/341