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Date of Award
2020
Document Type
Restricted Thesis: Campus only access
Degree Name
Bachelor of Science
Department
Biology
First Advisor
Dr. Angie Hilliker
Abstract
Mtr4 is an essential RNA helicase associated with the nuclear exosome and aids in the processing and degradation of many RNAs. Current questions about Mtr4 surround how Mtr4 performs these two separate functions, as well as what regions of the protein affect said functions. Through the random mutagenesis of the C-terminal region of MTR4, we selected for conditional mutants that are phenotypically defective at high temperature to investigate the function of Mtr4 in vivo. To investigate the ability of the conditional mtr4 mutants in processing various RNAs, we used chemiluminescent northern blotting to demonstrate one conditional mutant’s defects in RNA processing and degradation. In order to probe for additional metabolic pathways affected by these mutants, we have begun a phenotypic microarray testing over 1400 different growth and stress conditions.
Recommended Citation
Isenhour, Anthony, "Investigating conditional mutations in MTR4: an RNA helicase associated with the nuclear exosome" (2020). Honors Theses. 1489.
https://scholarship.richmond.edu/honors-theses/1489