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Date of Award


Document Type

Restricted Thesis: Campus only access

Degree Name

Bachelor of Science


Biochemistry & Molecular Biol.


Ded1 is an ATP-dependent RNA helicase implicated in translation initiation. Ded1 is known to be required for the resolution of highly structured 5' UTRs in order for the 48S pre-initiation complex to bind and scan for the start codon. Ded1 has two roles in translation initiation. First, Ded1 assembles with eIF4F during mRNA activation to prime the mRNA. Then, Ded1 uses its ATP-dependent helicase activity to unwind the secondary structure of the 5'UTR and resolve barriers along the mRNA. It was previously found that Ded1 is methylated at four arginine residues (Erce et al., 2013). In this paper, the effect of methylation on Ded1 protein is evaluated through in vitro translation, with future plans to perform helicase assays and oligomerization assays