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Date of Award
Restricted Thesis: Campus only access
Bachelor of Science
Biochemistry & Molecular Biol.
Dr. Eugene Wu
Cyclobutane Pyrimidine Dimers (CPDs) are photolesions that form when UV-‐B irradiation causes adjacent thymine or cytosine bases to covalently bond. These structures can arrest DNA synthesis by replicative polymerases, as they induce local helical distortions in the template strand that inhibit nucleotide incorporation. The primary goal of this study was to gain better insight into the effects of thymine dimers (T^T) on DNA replication in a model polymerase. Here, we present kinetic studies along with crystal structures of the Bacillus DNA polymerase I large fragment in complex with a CPD-‐containing template and an incoming dNTP. Together, our results suggest a mechanism by which CPDs can inhibit DNA synthesis in Bacillus DNA pol.
Walsh, Amanda Rose, "Cyclobutane pyrimidine dimer blocks replication in bacillus dna polymerase" (2015). Honors Theses. 918.