Date of Award
Bachelor of Science
Biochemistry & Molecular Biol.
Dr. Jonathan Dattelbaum
Secondary metabolites continue to offer a promising source of novel chemical entities with therapeutic bioactivities. Our research aims to isolate secondary metabolites produced by non-ribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) constructs. A metagenomic library was created from the marine bacterium Pseudoalteromonas sp., which was isolated from the Chesapeake Bay sponge Halichondria bowerbanki. Using a functional screen for PPTase activity we were able to identify colonies that contained NRPS and PKS gene clusters. Cultures of this microbe have been produced and analyzed using a combination of chromatography and 1H, 13C, HMBC, HSQC, COSY, AND NOESY NMR spectra. We were able to isolate a potential partial product from one of these gene clusters. Further investigation will be needed to confirm the identity of this compound, and perform biological assays to screen for relevant therapeutic activity.
Craig, Connor, "An NMR-guided approach to the isolation of secondary metabolites from NRPS and PKS gene clusters" (2015). Honors Theses. 916.