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Date of Award


Document Type

Restricted Thesis: Campus only access

Degree Name

Bachelor of Arts



First Advisor

Dr. Richard Topham


Callinectes sapidus (blue crab) hemocyanin seems to retain oxygen binding capacity more effectively under disulfide-reducing conditions that Limulus and Cancer hemocyanins, for example. Disulfide bond reduction of Callinectes hemocyanins by dithiothreitol (DIT) was performed under a variety of conditions in order to assess this behavior. It was found that calcium helped maintain oxygen binding capacity, as did pH's of 7.0, 8.0, and 8.5, rather than the usual 9.0. Furthermore, the denaturing polyacrylamide gel electrophoresis (PAGE) confirmed suspicions that a conformational change that appears to be responsible for the loss of oxygen binding does not occur in the presence of calcium, lowered pH, or chloride ion. These experiments open the door for further characterization of the Callinectes hemocyanin.