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Date of Award


Document Type

Restricted Thesis: Campus only access

Degree Name

Bachelor of Science




The blue crab, Callinectes sapidus, is a euryhaline invertebrate inhabiting aquatic areas with continually varying salinity levels. During conditions of decreasing salinity, Callinectes exhibits increased blood ammonia concentrations and increased ammonia excretion proposed to be the result of ammoniogenesis in gill mitochondria. Isolation of the mitochondrial fraction, by differential centrifugation, was followed by incubation with amino acids from the four major categories. Mitochondria were also incubated with ADP, rotenone, and FCCP in the presence of the two ammoniogenic substrates, serine and glutamic acid. The results indicate that gill mitochondria from Callinectes sapidus are indeed ammoniogenic, particularly with glutamic acid as a substrate. Additionally, there appears to be a relationship between ammoniogenesis and ATP production.