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Date of Award
Restricted Thesis: Campus only access
Bachelor of Science
Dr. Valerie M. Kish
Heat shock proteins (hsp) are specialized proteins that are synthesized by many organisms as a response to stressful conditions. HSP70 is a specific heat shock protein that targets damaged proteins to the proteasome for degradation. This 70kDa hsp has been identified in Chlamydomonas reinhardtii under conditions of heat shock. Chlamydomonas reinhardtii was exposed to conditions of oxidative stress using H2O2 and menadione and the resulting proteins were analyzed to identify a similar 70kDa hsp under oxidative stress. A monoclonal antibody was used to determine if the same 70kDa heat shock protein was produced under heat and oxidative stress. A 70kDa hsp was identified on an autoradiogram in heat stressed cells of Chlamydomonas reinhardtii, but no heat shock proteins were identified in the cells exposed to oxidative shock of H2O2 or menadione.
Dise, Rebecca S., "Heat shock proteins produced by Chlamydomonas reinhardtii under conditions of heat and oxidative stress" (1999). Honors Theses. 425.