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Author

Claire Brown

Date of Award

2020

Document Type

Restricted Thesis: Campus only access

Degree Name

Bachelor of Science

Department

Chemistry

First Advisor

Dr. Michelle Hamm

Abstract

Reactive oxygen species (ROS) can react with 2’-deoxyguanosine (dG) to create the promutagenic lesion 8-oxo-2’-deoxyguanosine (OdG). Changes in the sterics and electronics in the base of OdG, as compared to dG, allow it to base pair with both 2’-deoxycytidine (dC) and 2’-deoxyadenosine (dA). The latter pairing can lead to transversion mutations, which have been linked to aging, and diseases such as cancer, arthritis, and lupus. The potential for OdG to pair with dA during replication was previously investigated for the A-family polymerase, Klen-Taq (KT), which is a form of pol I from Thermus aquaticus. This study investigated the incorporation of dATP and dCTP across from dG, OdG, and seven of their analogues that differed in substituent size and hydrogen-bonding ability, and revealed that KT does not exhibit typical incorporation patterns opposite two sulfur-based analogues: 8-thio-2’-deoxyguanosine (SdG) and 8-thio-2’-deoxyinosine (SdI).

Available for download on Wednesday, May 07, 2025

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