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A carbon nanotube-based, first generation, amperometric biosensing system has been developed for the detection of sarcosine, a naturally ocurring molecule that, if present in high concentration in the urine, has been assocaited with prostrate cancer. Using layer-by-layer assembly, platinum electrodes were modified with carbon nantube doped chitosan films, immobilized sarcosine oxidase, and a stabilizing Nafion membrane before being subjected to standardized exposures of sarcosine under amperometric control.