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Author

Tess Munoz

Date of Award

2018

Document Type

Restricted Thesis: Campus only access

Degree Name

Bachelor of Science

Department

Chemistry

Abstract

A first-generation amperometric galactose biosensor is present. The sensor utilized xerogels as immobilization matrices for the galactose oxidase (GAOx) enzyme. Several parameters (xerogel silane precursor, buffer pH, enzyme concentration, drying time, and the inclusion of a polyurethane (PU) outer layer) were investigated for their effect on biosensor performance. Of those investigated, it was found that xerogel structure and the inclusion of an outer PU layer had the greatest effect on important sensing attributes such as limit of detection, response time, and linear sensing range.

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