DOI
10.17912/micropub.biology.001764
Author ORCID Identifier
Omar. A. Quintero-Carmona: https://orcid.org/0000-0002-9314-1704
Abstract
Myosin-X (MYO10) is an actin-based motor protein involved in cytoskeletal dynamics, membrane interactions, and integrin-mediated adhesion. To investigate MYO10's cellular roles, we generated MYO10 knockdown (MYO10KD) HeLa and COS7 cell lines using lentiviral shRNA. Compared to wild-type cells, both MYO10KD lines showed reduced proliferation and impaired cell migration in wound assays. Additionally, there were fewer edge filopodia in HeLa cells. Furthermore, MYO10KD cells demonstrated increased spreading on laminin-coated substrates, suggesting altered integrin activation and cytoskeletal linkage. Our results reinforce MYO10’s importance in cell proliferation, adhesion, and migration. These MYO10KD lines provide an accessible cell culture model for future study of MYO10.
Document Type
Article
Publication Date
9-19-2025
Publisher Statement
© 2025 by the authors. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Recommended Citation
Mas JA, Cristella CE, Phan VHMN, Wendt LS, Rose CA, Ali A, et al., Quintero-Carmona OA. 2025. Cells stably expressing shRNA against MYO10 display altered cell motility. microPublication Biology. 10.17912/micropub.biology.001764.
