CDX4 Regulates the Progression of Neural Maturation in the Spinal Cord.
DOI
10.1016/j.ydbio.2019.02.014
Abstract
The progression of cells down different lineage pathways is a collaborative effort between networks of extracellular signals and intracellular transcription factors. In the vertebrate spinal cord, FGF, Wnt and Retinoic Acid signaling pathways regulate the progressive caudal-to-rostral maturation of neural progenitors by regulating a poorly understood gene regulatory network of transcription factors. We have mapped out this gene regulatory network in the chicken pre-neural tube, identifying CDX4 as a dual-function core component that simultaneously regulates gradual loss of cell potency and acquisition of differentiation states: in a caudal-to-rostral direction, CDX4 represses the early neural differentiation marker Nkx1.2 and promotes the late neural differentiation marker Pax6. Significantly, CDX4 prevents premature PAX6-dependent neural differentiation by blocking Ngn2activation. This regulation of CDX4 over Pax6 is restricted to the rostral pre-neural tube by Retinoic Acid signaling. Together, our results show that in the spinal cord, CDX4 is part of the gene regulatory network controlling the sequential and progressive transition of states from high to low potency during neural progenitor maturation. Given CDX well-known involvement in Hox gene regulation, we propose that CDX factors coordinate the maturation and axial specification of neural progenitor cells during spinal cord development.
Document Type
Article
Publication Date
5-15-2019
Publisher Statement
Copyright © 2019, ScienceDirect.
Recommended Citation
Joshi, Piyush, Andrew J. Darr, and Isaac Skromne. “CDX4 Regulates the Progression of Neural Maturation in the Spinal Cord.” Developmental Biology 449, no. 2 (May 15, 2019): 132–42. https://doi.org/10.1016/j.ydbio.2019.02.014.