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Date of Award

4-29-1996

Document Type

Restricted Thesis: Campus only access

Degree Name

Bachelor of Science

Department

Biology

First Advisor

Dr. John Bishop

Abstract

The efficacy of a fiber optic probe, consisting of a sealed lipid-filled semi-permeable membrane bag attached to a fiber optic, was evaluated to determine the fiberprobe's utility as an indicator of bioconcentration of polyaromatic hydrocarbons (PARs) in biological lipids. Fluorometric analysis of PAR concentrations in the fiberprobe were compared to bare-fiber analysis of lipid extracts from Notemigonus crysoleucas fish tissue. Both fish and fiberprobes were exposed to various concentrations of the PAH 9-methylanthracene over a seven-day period. Correlations between concentrations in the fiberprobe and in the fish tissue were ascertained. The results of fiberprobe analysis will allow for development of a prediction curve for fish tissue concentration of PARs in situ when fiberprobes are deployed for specified periods of time.

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