Date of Award
Master of Science
Valerie M. Kish
Overexpression of matrix metalloproteinase 2, MMP-2, is correlated with highgrade gliomas in vivo. The purpose of this study was to investigate whether MMP-2 overexpression in glioma cell lines leads to increased invasion of glioma cells in vitro. The entire MMP-2 gene was isolated from U87, a glioma cell line known to have high expression of wild-type MMP-2. This :tvIMP-2 gene was inserted in a sense and antisense orientation into pCR3 .1 vectors with a CMV promoter to ensure high levels of expression. Glioma cell lines were transfected with the sense and antisense constructs, as well as the pCR3.l vector alone to serve as a control. Zymography and Western blot analysis of conditioned media taken from glioma cell lines U251, T98, and LNZ-308 transfected with sense MMP-2 revealed an overproduction ofMMP-2 relative to controls. The invasiveness of these transfected cell lines was evaluated using an in vitro invasion assay. These assays revealed significantly more invasion by cells that were overproducing MMP-2 relative to cells containing either vector alone (control) or vector containing the antisense construct. These results support the hypothesis that glioma cell lines overexpressing MMP2 are more invasive in vitro.
Sauers, Nicole Danielle, "The role of matrix metalloproteinase-2 in glioma invasion" (2001). Master's Theses. 902.