Date of Award
Master of Science
Adult male ICR mice of proven fertility were intraperitoneally injected daily with 60 mg/kg of 5-thio-D-glucose for 28 days. Body weights of treated male mice were unaffected during the experimental period. Relative testicular weights were significantly reduced at 121 days after initial injections in 5-thio treated males, but no significant decrease was observed at 28 days.
Onset of sterility was estimated to occur at approximately 4 weeks and persisted for 5 to 6 weeks after cessation of treatment at which time fertility was restored.
Beginning with the first injections and continuing for 121 days, the males were mated with untreated ICR female mice of proven fertility. Gross examinations of mouse embryos and fetuses were made at 18 days of gestation. It was repeatedly shown that there was a significant decrease in mean litter size in both pre- and post- sterility periods. Only minor fetal anomalies were found in treated and control groups. No significant difference was observed in frequencies of malformations or resorptions. However, in the post-sterility period, a significant decrease in normals and an increase in dead fetuses were observed.
Histological examination of 5-thio treated and control testes was made at 28 and 121 days. Testes of treated mice at 28 days revealed giant multinucleated cells and a number of seminiferous tubules with no spermatids or mature sperm. At 121 days, testes of treated males had only partially recovered. However, many normal tubules with mature sperm were evident.
Results from this study indicate that 5-thio-D-glucose does cause temporary sterility in male ICR mice and that the compound may be mutagenic.
McManaway, Mary E., "Reproductive and mutagenic effects of 5-thio-D-glucose injections in male mice, Mus musculus, ICR strain" (1980). Master's Theses. 717.