Date of Award
Master of Science
A study was undertaken to isolate and characterize by thin-layer chromatography the poisonous principle produced by the freshwater bryozoan, Lophopodella carteri (Hyatt).
The bryozoan homogenate was divided into a lipid and non-lipid portion and each was assayed for toxicity. Only the lipid extract was lethal to fishes. The lipid part was divided into six classes by thin-layer chromatography. When tested, four of the six classes were toxic. Thin layer chroatography was used to subdivide each class into fractions. Assays showed toxicity in nine of fourteen fractions.
Evidence indicates that the poisonous princi ple is a lipid and the large number of toxic lipid fractions suggests that it is a multi-component toxin.
Dolan, John Michael, "A determination by thin-layer chromatography of the chemical nature of the poison produced by the bryozoan lophopodella carteri (Hyatt)" (1971). Master's Theses. 329.