Date of Award

Spring 1971

Document Type


Degree Name

Master of Science




A study was undertaken to isolate and characterize by thin-layer chromatography the poisonous principle produced by the freshwater bryozoan, Lophopodella carteri (Hyatt).

The bryozoan homogenate was divided into a lipid and non-lipid portion and each was assayed for toxicity. Only the lipid extract was lethal to fishes. The lipid part was divided into six classes by thin-layer chromatography. When tested, four of the six classes were toxic. Thin­ layer chroatography was used to subdivide each class into fractions. Assays showed toxicity in nine of fourteen fractions.

Evidence indicates that the poisonous princi ple is a lipid and the large number of toxic lipid fractions suggests that it is a multi-component toxin.

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