Date of Award


Document Type


Degree Name

Master of Science




The object or this work was to develop a procedure for the quantitative determination of solanesol in the petroleum ether extract of tobacco. The amount of tobacco extracted into petroleum ether is routinely used to differentiate tobacco types. The extraction procedures have been extensively studied by the Association of Official Agricultural Chemists (AOAC) and reported in the United States Department of Agriculture (USDA) Technical Bulletin # 1186.

There is at present no method for the quantitative determination of solanesol. One procedure which comes closest, involves an infrared measurement of the tobacco extract. Because of the presence of other bands, the results are reported as "solanesol-like substances" rather than solanesol. The reported values are considerably higher than estimates obtained from large scale isolation of solanesol from tobacco.

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