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Date of Award
Restricted Thesis: Campus only access
Bachelor of Science
Biochemistry & Molecular Biol.
Dr. Krista Stenger
Vesicular monoamine transporter-1 (VMAT-1) is involved in transporting monoamines (serotonin, dopamine, norepinephrine) into storage vesicles within cells. Single nucleotide polymorphisms (SNPs) within the human VMAT-1 (hVMAT-1) gene SLC18A1 have been associated with neuropsychiatric disorders. This research project aims to better understand the biochemical and physiological effects of these polymorphisms on VMAT-1 protein expression and function. We used SDS-PAGE, western blotting, and a serotonin transport assay to show tha although VMAT-1 with a threonine at position 136 had no effect on protein expression, it reduced the protein’s monoamine transport activity by 20-25% when compared to the 136-Ile form. Studies have also begun to address the behavioral consequences of knocking out the VMAT-1 gene in mice. These results revealed a cognitive deficit in mice lacking the VMAT-1 gene. A biochemical assay has also demonstrated that young female VMAT-1 knockout mice have increased corticosterone (CORT) levels when compared to wild type mice. As the mice aged, this difference in CORT levels was no longer present.
Geng, Yingying, "Vesicular monoamine transporter-1: association with neuropsychiatric disorders" (2014). Honors Theses. 887.