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Date of Award


Document Type

Restricted Thesis: Campus only access

Degree Name

Bachelor of Science



First Advisor

Dr. Mittman


Genetic information is coded in the DNA of chromosomes within the nucleus of eukaryotic cells. In each case DNA exists as a long string of small subunits each closely associated with a histone protein core. These units are called nucleosomes and the histones involved are vital for normal functioning of protein synthesis and gene replication.

The actions of histone proteins on chromatin DNA are presently the interest of many areas of investigation. Many researchers have attempted to reveal the structural and functional relationships of histones with DNA, yet these remain the subject of much controversy.

The histone protein core exists as an octameric unit of consisting of four different histone proteins H2A, H2A, H3, and H4 (Busch, 1965). In studying these proteins it is noted that there is a significant conversation of amino acid sequences throughout the evolution of species (Kornberg, 1974). This stability of sequence is believed to stem from the need for these proteins to have highly conserved precise contacts with DNA and the other histones. Histone H4 is considered the most highly conserved protein in nature and is the subject of this specific investigation.