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Date of Award
Restricted Thesis: Campus only access
Bachelor of Science
Dr. Barbara A. Mittman
Biotin has been used a successful, non-radioactive nucleic acid hybridization prove to identify specific DNA sequences. We are using photobiotin, a photo-activated analogue of biotin, to label yeast DNA made in a plasmid to form a nucleic acid hybridization probe. Current labeling methods are hazardous and time consuming as they require radioactivity. Photobiotin is a more rapid method of labeling and will decrease risks associated with nucleic acid probe technology. The specific objective of our research is to verify the genetic make-up of yeast strains used in current laboratory research investigating histone mutations
Coston, JoAnna J., "The application of photobiotin as a non-radioactive genetic probe in Saccharomyces cerevisiae" (1989). Honors Theses. 449.