Off-campus University of Richmond users: To download campus access theses, please use the following link to log in to our proxy server with your university username and password.

Date of Award

Spring 1989

Document Type

Restricted Thesis: Campus only access

Degree Name

Bachelor of Science



First Advisor

Dr. Barbara A. Mittman


Biotin has been used a successful, non-radioactive nucleic acid hybridization prove to identify specific DNA sequences. We are using photobiotin, a photo-activated analogue of biotin, to label yeast DNA made in a plasmid to form a nucleic acid hybridization probe. Current labeling methods are hazardous and time consuming as they require radioactivity. Photobiotin is a more rapid method of labeling and will decrease risks associated with nucleic acid probe technology. The specific objective of our research is to verify the genetic make-up of yeast strains used in current laboratory research investigating histone mutations