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Date of Award


Document Type

Restricted Thesis: Campus only access

Degree Name

Bachelor of Science



First Advisor

Dr. Rafael de Sa


The evolutionary relationships of species within the frog family Microhylidae are as yet unresolved. Sequence data of the 28S nuclear rDNA gene of representatives of the family are needed to reconstruct this phylogeny, since morphological and biogeographical efforts are obscured by the diversity of the group.The research involved the cloning and sequencing of this gene for the genus Phrynomeru, the only representative of the subfamily Phrynomerinae. A sub-genomic library was constructed for the Phrynomerus bifasciatus, and the library was screened for rDNA clones. Positive clones were isolated and variable regions of the 28S subunit were sequenced using four available primers. The sequence data was aligned with X.laevis 28S rDNA gene using available sequences in Genbank. Four areas of the gene, involving 250 base pairs, have been sequenced and aligned. The availability of new primers will allow for the sequencing of other areas. In addition to Phrynomerus, a positive clone was also isolated and sequenced for the species Leptodactylus latinasus.