Utilizing the reverse transcriptase-polymerase chain reaction (RT-PCR) procedure, we have synthesized full-length copies of segment 10 from African horsesickness virus (AHSV) serotypes 1,4 and 8. The genes were cloned, sequenced and compared with the sequence of the cognate gene from AHSV serotypes 3 and 9. Sequences were analyzed to assess evolutionary relationships among serotypes using cladistics. Based on this analysis the data support a close relationship between serotypes 4 and 9 and between serotypes 1 and 8 and a closer relationship of serotype 3 to the 4 and 9 group.
Copyright © 1994, ScienceDirect.
de Sá, R. O. M. Zellner, and M. Grubman. 1994. Phylogenetic analysis of segment 10 from African Horsesickness Virus and cognate genes from other orbiviruses. Virus Research 33:157-165.