Date of Award
Master of Science
This study investigated whether rice exhibits exceptions to the accepted model, in cereals, for the induction of a and P-amylase in the embryo and endosperm during germination. We also tried to establish whether different patterns of induction were cultivar specific, or due to the influence of ripening temperature, or a component of the incubation medium. An isoelectric focusing technique was used to monitor a- and P-amylase activities, a spectrophotometric assay was used to quantify the activity of a-amylase, and two cDNA clones, RAmy3D and RAmylA, were used to measure a-amylase RNA accumulation by Northern analysis. Distinct patterns of induction of a-and P-amylase activity in the embryo and endosperm regions were determined to exist between cultivars, and they were not controlled by either the ripening temperature or a component of the incubation medium. We concluded that the observed differences were cultivar specific.
Mosley, Angela M., "Investigation of different patterns of induction of amylases in different cultivars of rice, Oryza sativa L." (1994). Master's Theses. 850.