Date of Award

5-1973

Document Type

Thesis

Degree Name

Master of Science

Department

Biology

Abstract

Pure cultures of the sporophyte and gametophyte generations of Allomyces arbusculus Butler (1911) were grown under varied conditions of temperature and pH. On solid media the gametophyte did not grow at 10 and 15 C; the sporophyte began slow growth after a long lag period at 15 C. There were no significant differences in growth between the sporophyte and gametophyte at 20 and 25 C. Increasing colony areas occurred for both generations at temperatures between 30 and 40 C, with the largest amount of growth for either generation at 35 C.

Colonies grown in broth media at various pH levels pro­ duced a larger amount of growth for both generations at all temperatures when the pH was periodically readjusted to the original level during incubation than when there was no read­ justment. The optimum pH for both generations with readjusted pH was 7.0; the optimum temperature was 32-37 C. The sporophyte demonstrated a significant difference in the amount of growth as compared to the gametophyte in both readjusted and unadjusted pH conditions.

Chemical analysis of both generations for total nitrogen was done with the Biuret test for protein and total carbohydrates with the anthrone reagent. The differences in the amount of total protein for both generations proved statistically nonsignificant with a slight increase in protein for both generations at pH 7.0 at temperatures of 27 and 32 C. Results of tests for total carbohydrate for both generations was variable with no definite conclusions being established.

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