Date of Award
5-1973
Document Type
Thesis
Degree Name
Master of Science
Department
Biology
Abstract
Previous attempts to measure the (Na++K+) - dependent ATPase in gills of euryhaline teleosts have yielded controversial results. In this study both the (Na++K+) - dependent ATPase activity and the number of JH - ouabain binding sites, representing the number of ATPase molecules, were measured in gill tissue preparations of the killifish, Fundulus heteroclitus. The (Na++K+) dependent ATPase activity and ouabain binding sites were greater in gills of fish fully adapted to freshwater than in those adapted to salinities of 17 ppt or 24 ppt. On the other hand, in 30 ppt salinity the (Na++K+) - dependent ATPase activity and ouabain binding sites were greater than in freshwater samples. Upon transfer of fish from freshwater to salt water (17 ppt) the (Na++K+)- dependent ATPase activity declined significantly within one half hour to a value which remained constant in salt water (17 ppt). Observed changes in (Na++K+) - dependent ATPase activity were not correlated with any significant changes in the Mg++ - dependent ATPase activity.
Recommended Citation
Gilman, Mary Elizabeth, "Changes in the (Na+ K+) - dependent ATPase activity in gills of the euryhaline teleost, Fundulus heteroclitus, upon adaptation to freshwater and salt water as determined by specific activities and 3H - ouabain binding sites" (1973). Master's Theses. 468.
https://scholarship.richmond.edu/masters-theses/468