Date of Award
Master of Science
Mycelial, sporangiophore, and zygospore solubilized protein fractions o Phycomyces blakesleeanus Burgeff were separated on polyacrylamide gels and stained. Protein patterns of 8 and 13 day old mycelia of the + strain were identical as were the patterns of the same two age groups of the - strain. The + mycelia exhibited 9 protein bands while the - strain contained 10 bands. Seven bands were homologous to both strains. Sporangiophore samples of 5 and 10 day old cultures from + and - strains contained 4 protein bands homologous to all 4 samples. The protein band at 9 mm was also homologous to all mycelial samples. The zygospore preparation yielded 2 protein bands. The band at 9 mrn in this preparation was homologous with all mycelial and sporangiophore samples, while the band at 7 mm was unique to only the zygospore sample.
Gillespie, Douglas Burton, "A comparative study of solubilized proteins from phycomyces blakesleeannus by disc electrophoresis" (1971). Master's Theses. 338.