"A comparative study of solubilized proteins from phycomyces blakesleea" by Douglas Burton Gillespie

Date of Award

Summer 1971

Document Type

Thesis

Degree Name

Master of Science

Department

Biology

Abstract

Mycelial, sporangiophore, and zygospore solubilized protein fractions o Phycomyces blakesleeanus Burgeff were separated on polyacrylamide gels and stained. Protein patterns of 8 and 13 day old mycelia of the + strain were identical as were the patterns of the same two age groups of the - strain. The + mycelia exhibited 9 protein bands while the - strain contained 10 bands. Seven bands were homologous to both strains. Sporangio­phore samples of 5 and 10 day old cultures from + and - strains contained 4 protein bands homologous to all 4 samples. The protein band at 9 mm was also homologous to all mycelial samples. The zygospore preparation yielded 2 protein bands. The band at 9 mrn in this preparation was homologous with all mycelial and sporangiophore samples, while the band at 7 mm was unique to only the zygospore sample.

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Biology Commons

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