Author

Jennie Altman

Date of Award

Spring 2013

Document Type

Thesis

Degree Name

Bachelor of Science

Department

Biology

First Advisor

Dr. Eugene Wu

Abstract

Due to the inherently error-prone nature of RNA replication, mutations to genomes of RNA viruses occur frequently and accumulate. We hypothesized that RNA versions of nucleoside analogues that increase mutation rates in DNA could cause increases in the mutation rate of a model RNA virus, Reovirus, and decrease the fitness of the virus in vitro due to the accumulation of deleterious mutations. After conducting multiple passages of Reovirus in mouse L929 cells in the presence of these potential RNA mutagens in four separate trials, there were not only virus samples with the expected decreased infectivity, but surprisingly, samples with marked increases in infectivity. The life histories of these Reoviruses under mutagenesis may clarify likely consequences of mutagenic antiviral therapy.

Included in

Biology Commons

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