DOI
10.1021/bi992997s
Abstract
The kynurenine pathway catabolite 3-hydroxykynurenine (3HK) and redox-active metals such as copper and iron are implicated in cataractogenesis. Here we investigate the reaction of kynurenine pathway catabolites with copper and iron, as well as interactions with the major lenticular structural proteins, the R-crystallins. The o-aminophenol kynurenine catabolites 3HK and 3-hydroxyanthranilic acid (3HAA) reduced Cu(II)>Fe(III) to Cu(I) and Fe(II), respectively, whereas quinolinic acid and the nonphenolic kynurenine catabolites kynurenine and anthranilic acid did not reduce either metal. Both 3HK and 3HAA generated superoxide and hydrogen peroxide in a copper-dependent manner. In addition, 3HK and 3HAA fostered copper-dependent R-crystallin cross-linking. 3HK- or 3HAA-modifed R-crystallin showed enhanced redox activity in comparison to unmodified R-crystallin or ascorbate-modified R-crystallin. These data support the possibility that 3HK and 3HAA may be cofactors in the oxidative damage of proteins, such as R-crystallin, through interactions with redox-active metals and especially copper. These findings may have relevance for understanding cataractogenesis and other degenerative conditions in which the kynurenine pathway is activated.
Document Type
Article
Publication Date
2000
Publisher Statement
Copyright © 2000, American Chemical Society.
DOI: https://doi.org/10.1021/bi992997s
The definitive version is available at: https://pubs.acs.org/doi/10.1021/bi992997s
Recommended Citation
L.E. Goldstein, M.C. Leopold, X. Huang, C.S. Atwood, A.J. Saunders, M. Hartshorn, K.Y. Faget, J.A. Muffat, R.C. Scarpa, L.T. Chylact, J.T. Lim, E.F. Bowden, R.E. Tanzi and A. Bush. “3-Hydroxykynurenine and 3-Hydroxyanthranilic Acid Generate Hydrogen Peroxide and Promote α-Crystallin Cross Linking by Metal Ion Reduction,” Biochemistry 2000, 39, 7266-7275.
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