Reconstructing three dimensional structures (3DR) from histological sections has always been difficult but is becoming more accessible with the assistance of digital imaging. We sought to assemble a low cost system using readily available hardware and software to generate 3DR for a study of tadpole chondrocrania. We found that a combination of RGB camera, stereomicroscope, and Apple Macintosh PowerPC computers running NIH Image, Object Image, Rotater. and SURFdriver software provided acceptable reconstructions. These are limited in quality primarily by the distortions arising from histological protocols rather than hardware or software.
Copyright © 1999 Virginia Academy of Science. This article first appeared in Virginia Journal of Science 50, no. 3 (Fall 1999): 227-35.
Please note that downloads of the article are for private/personal use only.
Radice, Gary, Mary Kate Boggiano, Mark DeSantis, Peter Larson, Joseph Opping, Matthew Smetanick, Todd Stevens, James Tripp, Rebecca Weber, Michael Kerckhove, and Rafael O. de Sá. "Three-Dimensional Reconstructions of Tadpole Chondrocrania from Histological Sections." Virginia Journal of Science 50, no. 3 (Fall 1999): 227-35.